She was voiced by the late Phyllis Diller.
Prior to Atta coming of age, The Queen handled all the colony's food production oversight for the Grasshoppers as a form of protection/extortion. Due to an accident caused by Flik the excess food is destroyed before the Grasshoppers arrive and Atta is assumed an incompetent Queen compared to her mother. Despite the Queen's attempts to smooth things over The Grasshoppers place an unreasonable ultimatum on the colony that would leave them unable to gather food for themselves before winter sets in. Though The Queen plays little more than a side character in the film she is still a living Queen and seen by the colony as the center of their universe. The Queen's main contribution from the beginning of the film is strictly in an advisory fashion to her daughter as Atta tries to cope with the stresses and responsibilities of leadership.
At the end of the film, the Queen retires and Atta becomes the new queen with Dot becoming the heir to the throne.
- The Queen is similar to The Queen Ant from Dreamworks' Antz, as both are ants and both are the leaders of their colony. Both are also the mother of the princess of the colony (Atta and Bala) and later become the mother-in-law of the protagonist (Flik and Z).